Hattori T, Hoffman T, Hirata F
Biochem Biophys Res Commun. 1983 Mar 16;111(2):551-9. doi: 10.1016/0006-291x(83)90342-x.
When U 937 cells, a human histiocytic lymphoma cell line, were cultured with purified lipomodulin for 3 days, morphological and functional differentiation was induced as detected by microscopical examination of Giemsa stained smears, expression of mature monocyte antigen, and antibody dependent cellular cytotoxicity tests. Essentially similar differentiation was observed by the treatment with dexamethasone for 6 days and this differentiation by dexamethasone was blocked by monoclonal anti-lipomodulin antibody. Furthermore, the synthesis of immunoprecipitable lipomodulin in these cells was induced by dexamethasone treatment. These results, taken together, suggest that the induction of lipomodulin synthesis might be the primary event in dexamethasone-induced cellular differentiation of U 937 cells.
当人组织细胞淋巴瘤细胞系U937细胞与纯化的脂调素一起培养3天时，通过吉姆萨染色涂片的显微镜检查、成熟单核细胞抗原的表达以及抗体依赖性细胞毒性试验检测到诱导了形态和功能分化。用 dexamethasone 处理6天观察到基本相似的分化，并且 dexamethasone 诱导的这种分化被单克隆抗脂调素抗体阻断。此外，地塞米松处理可诱导这些细胞中可免疫沉淀的脂调素的合成。综合这些结果表明，脂调素合成的诱导可能是地塞米松诱导U937细胞分化的主要事件。
