Liem H H, Johnson E F, Muller-Eberhard U
Biochem Biophys Res Commun. 1983 Mar 29;111(3):926-32. doi: 10.1016/0006-291x(83)91388-8.
Rabbits treated with phenobarbital were given a single injection of allylisopropylacetamide (AIA) s.c. and/or heme i.v. Hepatic microsomes were isolated 1, 5 and 24 hours post injection and the microsomal contents of both total cytochrome P-450 chromophore, and the protein moiety of P-450 2 were determined by spectrophotometric and immunochemical methods respectively. AIA caused the levels of total P-450 chromophore and of P-450 2 protein to decline to 30% of the control values at 5 hours post-injection. Concurrent administration of heme with AIA diminished the decrease in the total microsomal content of P-450 chromophore but not in that of P-450 2 protein. These findings suggest that the destruction of the heme prosthetic group of P-450 by suicide substrates such as AIA may lead to an enhanced degradation of the apo-P-450.
给用苯巴比妥治疗的兔子皮下注射一次烯丙异丙基乙酰胺（AIA）和/或静脉注射血红素。在注射后1、5和24小时分离肝微粒体，分别用分光光度法和免疫化学方法测定总细胞色素P-450发色团和P-450 2蛋白部分的微粒体含量。AIA导致注射后5小时总P-450发色团和P-450 2蛋白水平降至对照值的30%。血红素与AIA同时给药减少了P-450发色团微粒体总含量的下降，但没有减少P-450 2蛋白的下降。这些发现表明，自杀性底物如AIA对P-450血红素辅基的破坏可能导致脱辅基P-450的降解增强。
