Spudich E N, Bogomolni R A, Spudich J L
Biochem Biophys Res Commun. 1983 Apr 15;112(1):332-8. doi: 10.1016/0006-291x(83)91835-1.
Retinal-binding proteins in bacteriorhodopsin-deficient membranes of Halobacterium halobium have been separated on polyacrylamide gels after labeling with (3H)retinal. Four labeled polypeptides are observed: one at an Mr of 19,000, two distinct bands near 25,000, and one at 94,000. Comparing halorhodopsin-containing and halorhodopsin-deficient mutants and using ion translocation measurements and flash spectroscopy to test for the photoactive retinal pigments, we show that the lower of the two radiolabeled bands at 25,000 is the retinal-binding polypeptide of halorhodopsin. The identities of the other three polypeptides are not known, but one of them, probably the upper of the two 25,000 bands, is likely to be s-rhodopsin.
在用（3H）视黄醛标记后，嗜盐菌紫膜缺失细菌视紫红质的膜中的视网膜结合蛋白已在聚丙烯酰胺凝胶上分离。观察到四条标记多肽：一条分子量为19,000，两条在25,000附近的不同条带，一条在94,000。比较含嗜盐视紫红质和缺失嗜盐视紫红质的突变体，并使用离子转运测量和闪光光谱法测试光活性视网膜色素，我们发现25,000处两条放射性标记条带中较低的一条是嗜盐视紫红质的视网膜结合多肽。其他三条多肽的身份未知，但其中一条，可能是25,000两条条带中较高的那条，可能是s-视紫红质。
