盐对嗜盐菌视紫红质及第三种类视紫红质色素光循环和离子泵浦的影响。
Effect of salt on photocycle and ion-pumping of halorhodopsin and third rhodopsinlike pigment of Halobacterium halobium.
作者信息
Hazemoto N, Kamo N, Kobatake Y, Tsuda M, Terayama Y
出版信息
Biophys J. 1984 Jun;45(6):1073-7. doi: 10.1016/S0006-3495(84)84254-X.
Abstract
The cytoplasmic membranes of Halobacterium halobium contain at least three retinal pigments: bacteriorhodopsin (bR), halorhodopsin (hR), and a third rhodopsinlike pigment (tR). The amplitudes of the phototransient in the photolysis of hR and tR were measured in various salt solutions. Halogen ion (except fluoride) was required to retain the photocycle of hR. Parallels between the amplitude of the phototransient of hR and the magnitude of the photo-induced tetraphenylphosphonium (TPP+) uptake suggests that hR is a light-driven halogen pump, which supports the hypothesis by Schobert and Lanyi (J. Biol. Chem., 1982, 257:10306-10313). The order of effectiveness of halogen was Br- greater than Cl- greater than I-. On the other hand, no specific ion was required to retain the photocycle of tR, and tR was concluded to be nonelectrogenic.
摘要
嗜盐菌的细胞质膜含有至少三种视黄醛色素
细菌视紫红质（bR）、嗜盐视紫红质（hR）和第三种视紫红质样色素（tR）。在各种盐溶液中测量了hR和tR光解过程中的光瞬变幅度。卤素离子（氟除外）是维持hR光循环所必需的。hR光瞬变幅度与光诱导四苯基鏻（TPP+）摄取量之间的平行关系表明hR是一种光驱动的卤素泵，这支持了Schobert和Lanyi（《生物化学杂志》，1982年，257:10306 - 10313）的假设。卤素的有效性顺序为Br-＞Cl-＞I-。另一方面，维持tR的光循环不需要特定离子，并且得出tR是非电生的结论。
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