双层脂质膜的光子相关光谱法。
Photon correlation spectroscopy of bilayer lipid membranes.
作者信息
Crilly J F, Earnshaw J C
出版信息
Biophys J. 1983 Feb;41(2):197-210. doi: 10.1016/S0006-3495(83)84420-8.
Light scattering by thermal fluctuations on simple monoglyceride bilayer membranes has been used to investigate the viscoelastic properties of these structures. Spectroscopic analysis of these fluctuations (capillary waves) permits the nonperturbative measurement of the interfacial tension and a shear interfacial viscosity acting normal to the membrane plane. The methods were established by studies of solvent and nonsolvent bilayers of glycerol monooleate (GMO). Changes in the tension of GMO/n-decane membranes induced by altering the composition of the parent solution were detected and quantified. In a test of the reliability of the technique controlled variations of the viscosity of the aqueous bathing solution were accurately monitored. The technique was applied to solvent-free bilayers formed from dispersions of GMO in squalane. The lower tensions observed attested to the comparative absence of solvent in such bilayers. In contrast to the solvent case, the solvent-free membranes exhibited a significant transverse shear viscosity, indicative of the enhanced intermolecular interactions within the bilayer.
利用简单甘油单酯双层膜上热涨落引起的光散射来研究这些结构的粘弹性特性。对这些涨落（毛细波）进行光谱分析，可以对界面张力以及垂直于膜平面作用的剪切界面粘度进行无扰动测量。这些方法是通过对甘油单油酸酯（GMO）的溶剂双层和非溶剂双层的研究而建立的。检测并量化了通过改变母液组成引起的GMO/正癸烷膜张力的变化。在该技术可靠性测试中，准确监测了水浴溶液粘度的可控变化。该技术应用于由GMO在角鲨烷中的分散体制备的无溶剂双层膜。观察到的较低张力证明了此类双层膜中溶剂相对较少。与有溶剂的情况相反，无溶剂膜表现出显著的横向剪切粘度，这表明双层膜内分子间相互作用增强。
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