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通过空间自协方差对生物膜进行定量表征。

Quantitative characterization of a biological membrane by means of its spatial autocovariance.

作者信息

Rasigni G, Palmari J, Rasigni M, Varnier F, Palmari J P, Marty F, Llebaria A

出版信息

Biophys J. 1985 Mar;47(3):431-6. doi: 10.1016/S0006-3495(85)83934-5.

Abstract

Profiles for the exoplasmic face (EF) of the freeze-fractured plasma membrane from the root storage tissue of red beets are reconstructed by microdensitometry of micrographs of surface-shadowed-platinum carbon replicas. Autocovariance functions (ACFs) are computed from those profiles. The initial portions of the ACFs have a Gaussian form whose parameters (root mean square surface roughness and autocovariance length) are estimated. The parameter estimates are used to show that the pits on the EF faces are in good complementarity with the intramembrane particles seen on the complementary protoplasmic fracture faces.

摘要

通过对表面阴影铂碳复制品的显微照片进行显微密度测定,重建了红甜菜根贮藏组织冷冻断裂质膜外质面(EF)的轮廓。自协方差函数(ACF)由这些轮廓计算得出。ACF的初始部分具有高斯形式,其参数(均方根表面粗糙度和自协方差长度)被估计出来。这些参数估计值用于表明EF面上的凹坑与在互补原生质断裂面上看到的膜内颗粒具有良好的互补性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beb2/1435201/87902dbcea14/biophysj00194-0169-a.jpg

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