Speek A J, Odink J, Schrijver J, Schreurs W H
Clin Chim Acta. 1983 Feb 28;128(1):103-13. doi: 10.1016/0009-8981(83)90060-8.
A reliable high-performance liquid chromatographic method is presented for the determination of the urinary free catecholamines noradrenaline, adrenaline and dopamine. Urine is purified on a column of immobilized boric acid. Catecholamines are separated by ion-pair reversed phase high-performance liquid chromatography and detected electrochemically. The method is suited for routine analysis. It allows the determination of urinary free catecholamines in concentrations as low as 1 microgram/1 for noradrenaline and adrenaline and 5 micrograms/1 for dopamine. A single analysis can be completed within 1 h. Routine analyses can be carried out in a series of 40 samples within 2 days. The within-assay and between-assay coefficients of variation of the analyses in urine were both 2.9% for noradrenaline, both 5.0% for adrenaline, and 1.9 and 2.1% for dopamine. The chromatographic properties of the immobilized boric acid were investigated. In particular, the elution pattern of a series of catecholamine metabolites and analogues was determined. Under the conditions used, only basic compounds containing both a vicinal hydroxyl configuration and a primary or secondary amino group adsorb and elute together with the free catecholamines.
本文介绍了一种可靠的高效液相色谱法，用于测定尿中游离儿茶酚胺去甲肾上腺素、肾上腺素和多巴胺。尿液在固定化硼酸柱上进行纯化。儿茶酚胺通过离子对反相高效液相色谱法分离，并通过电化学检测。该方法适用于常规分析。它能够测定尿中游离儿茶酚胺，去甲肾上腺素和肾上腺素的最低检测浓度为1微克/升，多巴胺为5微克/升。一次分析可在1小时内完成。常规分析可在2天内对40个样品进行一系列检测。尿样分析中，去甲肾上腺素的批内和批间变异系数均为2.9%，肾上腺素均为5.0%，多巴胺分别为1.9%和2.1%。对固定化硼酸的色谱特性进行了研究。特别确定了一系列儿茶酚胺代谢物和类似物的洗脱模式。在所使用的条件下，只有同时含有邻位羟基构型和伯氨基或仲氨基的碱性化合物才会与游离儿茶酚胺一起吸附和洗脱。
