Jackman G P
Clin Chem. 1981 Jul;27(7):1202-4.
I describe a relatively simple and rapid procedure for quantitative analysis for unconjugated urinary norepinephrine, epinephrine, and dopamine. Catecholamines are isolated from urine on a carboxylic acid ion-exchange resin (Bio-Rex 70) and eluted with 0.8 mol/L boric acid. The eluates are assayed for the individual catecholamines by "high-performance" liquid chromatography with an octadecylsilane reversed-phase column and 10 mmol/L perchloric acid-acetonitrile (99/1) as the mobile phase. The catecholamines are detected by measuring their intrinsic fluorescence on excitation at 200 nm. The method is highly reproducible, the coefficient of variation for normal concentrations in urine being 4.4% for norepinephrine, 8.8% for epinephrine, and 4.8% for dopamine. Moreover, it appears to be practically free of interference by drugs or endogenous compounds in urine. The range of catecholamine concentrations found in 143 hypertensive patients agrees well with previous studies.
我描述了一种相对简单且快速的用于定量分析尿中游离去甲肾上腺素、肾上腺素和多巴胺的方法。儿茶酚胺通过羧酸离子交换树脂（Bio-Rex 70）从尿液中分离出来，并用0.8mol/L硼酸洗脱。洗脱液通过“高效”液相色谱法进行分析，使用十八烷基硅烷反相柱，以10mmol/L高氯酸 - 乙腈（99/1）作为流动相来测定各儿茶酚胺。通过在200nm激发波长下测量儿茶酚胺的固有荧光来进行检测。该方法具有高度可重复性，尿中正常浓度的变异系数去甲肾上腺素为4.4%，肾上腺素为8.8%，多巴胺为4.8%。此外，该方法似乎几乎不受尿液中药物或内源性化合物的干扰。在143例高血压患者中发现的儿茶酚胺浓度范围与先前的研究结果非常吻合。
