Van de Voorde J, Leusen I
Eur J Pharmacol. 1983 Jan 28;87(1):113-20. doi: 10.1016/0014-2999(83)90056-0.
Despite their potent vasodilating action in vivo, acetylcholine and histamine often show a vasoconstricting action in vitro. As the endothelium has an important role in the vasodilating effect of acetylcholine, we investigated the possible role of the endothelium in the vasodilating effect of histamine in comparison to acetylcholine. Experiments were done on ring segments of rat thoracic aorta mounted for isometric tension measurements. We demonstrated that relaxation by histamine and acetylcholine of pre-contracted rat aorta segments required the presence of endothelial cells. Acetylcholine acting on muscarinic receptors, and histamine acting on H1-receptors seemed to initiate the production of mediator(s) from the endothelial cells, which leads to relaxation of the vascular smooth muscle cells. This production appeared to be depressed by ETYA and hydroquinone, and under hypoxic conditions.
尽管乙酰胆碱和组胺在体内具有强大的血管舒张作用，但它们在体外常表现出血管收缩作用。由于内皮细胞在乙酰胆碱的血管舒张作用中起重要作用，我们研究了与乙酰胆碱相比，内皮细胞在组胺血管舒张作用中可能发挥的作用。实验采用安装好用于等长张力测量的大鼠胸主动脉环段进行。我们证明，组胺和乙酰胆碱使预收缩的大鼠主动脉段舒张需要内皮细胞的存在。作用于毒蕈碱受体的乙酰胆碱和作用于H1受体的组胺似乎启动了内皮细胞中介质的产生，从而导致血管平滑肌细胞舒张。在ETYA和对苯二酚存在的情况下以及在缺氧条件下，这种介质的产生似乎受到抑制。
