Wakade A R, Edgar D, Thoenen H
Exp Cell Res. 1983 Apr 1;144(2):377-84. doi: 10.1016/0014-4827(83)90417-2.
Neurons were dissociated from the sympathetic ganglia of embryonic chicks, and cultured in the absence of non-neuronal cells. Both nerve growth factor (NGF) and high concentrations of extracellular K+ supported neuronal survival, and these effects were independent of the presence of serum in the culture medium. Only 60% of the neurons survived in response to 35 mM K+, and survival was not increased when both NGF and K+ were present together. It was, however, possible to maintain essentially all the neurons in culture with either NGF or high K+ concentrations if the culture substrate had been pretreated with heart cell-conditioned medium (which did not itself support neuronal survival). These observations are consistent with a common mechanism of action of both K+ and NGF for the survival of cultured embryonic neurons.
从胚胎小鸡的交感神经节中分离出神经元，并在无非神经元细胞的条件下进行培养。神经生长因子（NGF）和高浓度的细胞外钾离子（K+）都能支持神经元的存活，而且这些作用与培养基中血清的存在无关。仅60%的神经元对35 mM K+有反应而存活，当NGF和K+同时存在时，存活率并未增加。然而，如果培养底物先用心脏细胞条件培养基预处理（其本身不支持神经元存活），那么用NGF或高K+浓度基本上可以维持培养中的所有神经元。这些观察结果与K+和NGF对培养的胚胎神经元存活的共同作用机制是一致的。
