Vasta G R, Warr G W, Marchalonis J J
Dev Comp Immunol. 1983 Winter;7(1):13-20. doi: 10.1016/0145-305x(83)90050-2.
We have detected and partially characterized multiple lectins present in the serum of the freshwater prawn Macrobrachium rosenbergii. Since agglutination of erythrocytes (RBC) is not abolished by treatment with Vibrio cholerae neuraminidase (VCN), Macrobrachium shows an agglutination pattern different from that of other sialic acid-specific lectins such as Limulus polyphemus lectin. However, after absorption with primate and bird VCN-treated RBC, Macrobrachium serum exhibits high titers with untreated and pronase-treated RBC and no agglutination of VCN-treated RBC, suggesting a typical sialic acid specific lectin agglutination profile. Hemagglutination-inhibition tests indicate that sialic acid containing compounds are the best inhibitors for Macrobrachium lectins. Subterminal sugars and type of linkage are probably important for the lectin binding since bovine submaxillary mucin (containing mainly terminal NANA-alpha-2 6-GalNAc-) is a better inhibitor than fetuin (containing mainly terminal NANA-alpha-2 leads to 3-Gal-) and colominic acid (-NANA-alpha-2 leads to 8-NANA-) is a weak inhibitor.
我们已经检测并部分鉴定了罗氏沼虾血清中存在的多种凝集素。由于用霍乱弧菌神经氨酸酶（VCN）处理后红细胞（RBC）的凝集并未被消除，罗氏沼虾表现出与其他唾液酸特异性凝集素（如鲎试剂凝集素）不同的凝集模式。然而，在用灵长类和鸟类经VCN处理的RBC吸收后，罗氏沼虾血清对未处理和经链霉蛋白酶处理的RBC表现出高滴度，而对经VCN处理的RBC无凝集，提示典型的唾液酸特异性凝集素凝集谱。血凝抑制试验表明，含唾液酸的化合物是罗氏沼虾凝集素的最佳抑制剂。末端下的糖和连接类型可能对凝集素结合很重要，因为牛颌下粘蛋白（主要含末端NANA-α-2,6-GalNAc-）是比胎球蛋白（主要含末端NANA-α-2,3-Gal-）更好的抑制剂，而共聚唾液酸（-NANA-α-2,8-NANA-）是弱抑制剂。
