Cassels F J, Marchalonis J J, Vasta G R
Comp Biochem Physiol B. 1986;85(1):23-30. doi: 10.1016/0305-0491(86)90216-6.
Callinectes sapidus serum and hemocyte microsomal fraction agglutinated a panel of untreated and enzyme treated vertebrate erythrocytes and cultured lymphoid cell lines. Crossed absorption experiments suggested the presence of multiple specific lectins in the serum. The microsomal fraction showed a 35-fold increase in specific activity when compared to the hemocyte lysate suggesting that hemocyte lectins are membrane-associated. Agglutination by serum and hemocyte lectins was inhibited by low concentrations of N-acylamino compounds including sialic, N-acetylmuramic and N-acetylglutamic acids, GalNAc, GlcNAc, ManNAc, and glycoproteins and polysaccharides which contain these carbohydrates: bovine submaxillary mucin, human orosomucoid, porcine stomach mucin and colominic acid. Hemagglutination by lectins of both serum and hemocyte microsomal fraction required divalent cations as suggested by the reduction in hemagglutination titer in the presence of the chelators EDTA, EGTA, CDTA and citrate.
美味蓝蟹血清和血细胞微粒体组分能凝集一组未经处理和经酶处理的脊椎动物红细胞以及培养的淋巴细胞系。交叉吸收实验表明血清中存在多种特异性凝集素。与血细胞裂解物相比，微粒体组分的比活性提高了35倍，这表明血细胞凝集素与膜相关。血清和血细胞凝集素引起的凝集反应可被低浓度的N-酰基氨基化合物抑制，这些化合物包括唾液酸、N-乙酰胞壁酸、N-乙酰谷氨酸、N-乙酰半乳糖胺、N-乙酰葡糖胺、N-乙酰甘露糖胺，以及含有这些碳水化合物的糖蛋白和多糖：牛颌下粘蛋白、人血清类粘蛋白、猪胃粘蛋白和聚唾液酸。血清和血细胞微粒体组分的凝集素引起的血细胞凝集反应都需要二价阳离子，这一点从螯合剂乙二胺四乙酸（EDTA）、乙二醇双四乙酸（EGTA）、环己烷二胺四乙酸（CDTA）和柠檬酸盐存在时血细胞凝集滴度降低可以看出。
