Kuznetzov A A, Goncharov B F, Burzawa-Gerard E
Gen Comp Endocrinol. 1983 Mar;49(3):364-74. doi: 10.1016/0016-6480(83)90201-0.
Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.
从星鲟（Acipenser stellatus Pall.）经丙酮处理的垂体中分离并纯化出促性腺激素的四种生物活性组分（aci-GTH-A、-B、-C、-D）。它们通过DEAE-纤维素色谱法进行分离。圆盘电泳，特别是聚丙烯酰胺凝胶等电聚焦显示，每个组分都包含几种成分。在四种aci-GTH制剂中，总共可以计数不少于15种不同的成分，其等电点范围为4.5至7.0。所有这些成分在蟾蜍卵母细胞成熟试验中均具有活性。四种制剂中只有两种（aci-GTH-A和-D）几乎不含共同成分。所有aci-GTH制剂在分子量、沉降系数、唾液酸含量和一些免疫学特性方面均显示为均一且相同。N端氨基酸分析显示，所有aci-GTH制剂中均含有酪氨酸和亮氨酸，唯一的例外是aci-GTH-D，它含有一种额外的N端为甘氨酸的多肽。当垂体提取物、新纯化的或保存3年的激素制剂进行等电聚焦时，未发现aci-GTH同工型光谱有差异。
