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[鲟鱼垂体促性腺激素的异质性]

[Heterogeneity of pituitary gonadotropin from sturgeons].

作者信息

Goncharov B F, Kuznetsov A A, Burzava-zherar E

出版信息

Biokhimiia. 1980 Mar;45(3):455-62.

PMID:7378484
Abstract

A highly purified preparation of the pituitary gonadotropin of a chondrostean fish, sevryuga (Acipenser stellatus Pall.) has been shown to be homogeneous by criteria of molecular weight, sedimentation coefficient and by N-terminal amino acid analysis. On the contrary, polyacrylamide gel electrophoresis and isoelectric fucusing have revealed a marked heterogeneity of the hormone preparation. The determination of gonadotropic activity (in vitro amphibian oocyte maturation test) has shown the similar specific activities of the differently charged hormone molecules.

摘要

一种软骨硬鳞鱼(星鲟,Acipenser stellatus Pall.)垂体促性腺激素的高度纯化制剂,通过分子量、沉降系数标准以及N端氨基酸分析表明其具有均一性。相反,聚丙烯酰胺凝胶电泳和等电聚焦显示该激素制剂存在明显的异质性。促性腺活性测定(体外两栖类卵母细胞成熟试验)表明,不同电荷的激素分子具有相似的比活性。

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