Thompson K V, Holliday R
Gerontology. 1983;29(2):73-82. doi: 10.1159/000213096.
Fibroblast cultures were established from skin biopsies from 4 male Werner's syndrome patients, aged 45-50. Several subcultures were obtained from each primary outgrowth and these were passaged to the end of their in vitro lifespan. Their average longevity was significantly less than control skin fibroblasts, but there was extreme variability amongst parallel cultures. In the most thoroughly studied case, the longevity of 19 subcultures derived from a single biopsy varied from 5 to 26 passages, showing that there was considerable heterogeneity in the growth potential of the cells in the primary culture. Moreover, the growth rate from any one culture was not uniform, since long periods of slow growth were sometimes succeeded by much more rapid proliferation. These features of Werner's syndrome fibroblast populations are not seen in cultures from normal individuals. The longevity of fibroblasts from one progeria patient was also shown to be much shorter than controls. Metaphases from 3 Werner's patients demonstrated a much higher frequency of chromosome abnormalities than in normal fibroblasts and also provided evidence of subclones containing a characteristic 'marker' chromosome.
从4名年龄在45 - 50岁的男性沃纳综合征患者的皮肤活检组织中建立了成纤维细胞培养物。从每个原代生长物中获得了几个传代培养物，并将它们传代至体外寿命结束。它们的平均寿命明显短于对照皮肤成纤维细胞，但平行培养物之间存在极大的变异性。在研究最深入的病例中，来自一次活检的19个传代培养物的寿命从5代到26代不等，表明原代培养物中细胞的生长潜力存在相当大的异质性。此外，任何一种培养物的生长速率都不均匀，因为长时间的缓慢生长有时会被更快的增殖所取代。沃纳综合征成纤维细胞群体的这些特征在正常个体的培养物中未见。一名早老症患者的成纤维细胞寿命也被证明比对照短得多。3名沃纳综合征患者的中期染色体显示出比正常成纤维细胞更高的染色体异常频率，并且还提供了含有特征性“标记”染色体的亚克隆的证据。
