通过凝胶色谱法从尿毒症超滤物中获得的乳酸脱氢酶抑制组分的等速电泳图谱。
Isotachophoretic pattern of LDH inhibiting fractions obtained from uremic ultrafiltrate by gelchromatography.
作者信息
De Smet R, van Landschoot N, van der Stiggel G, Ringoir S
出版信息
Int J Artif Organs. 1983 Mar;6(2):67-70.
PMID:6840893
Abstract
Uremic ultrafiltrate was fractioned by chromatography on Sephadex G15; two fractions were highly inhibiting the total lactate dehydrogenase activity in rat kidney homogenate. The inhibiting fractions were eluates number 10 and 11 with an elution volume of respectively 17.4 ml and 19.3 ml and a Kav of 0.17 and 0.25. Both fractions presented a different pattern on analysis by analytical isotachophoresis.
摘要
尿毒症超滤物通过在葡聚糖凝胶G15上进行色谱分离；两个级分对大鼠肾脏匀浆中的总乳酸脱氢酶活性具有高度抑制作用。抑制级分为洗脱体积分别为17.4 ml和19.3 ml、Kav分别为0.17和0.25的第10号和第11号洗脱液。通过分析等速电泳分析，这两个级分呈现出不同的图谱。
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