Prior R L
J Anim Sci. 1983 Feb;56(2):483-92. doi: 10.2527/jas1983.562483x.
An experiment was conducted to 1) evaluate the effects of diet (alfalfa hay vs high concentrate) on adipose tissue cellularity and rates of in vitro lipogenesis and 2) determine if there was a relationship between in vitro lipogenic rates from acetate and lactate and rates of L- or D-lactate disappearance from plasma. Number of adipose cells/g of tissue decreased with time on experiment; however, hay-fed steers had fewer, but larger cells/g of subcutaneous adipose tissue compared with concentrate-fed steers (.78 +/- .04 vs 1.20 +/- .13 X 10(-6)/g, respectively). These results, however, are likely due to a higher (approximately 25%) intake of dry matter and metabolizable energy by the hay-fed steers. Carcass data obtained at slaughter (460 kg) indicated that the concentrate-fed steers had as much or more adipose tissue compared with the hay-fed steers. Characteristics describing D- or L-lactate disappearance from plasma were not highly correlated with lactate utilization for fatty acid synthesis. Utilization of acetate as a substrate for fatty acid synthesis in vitro was correlated (r = .64) with the rate of lactate utilization for fatty acid synthesis.
进行了一项实验，以：1）评估日粮（苜蓿干草与高浓度精料）对脂肪组织细胞组成以及体外脂肪生成率的影响；2）确定乙酸盐和乳酸盐的体外脂肪生成率与血浆中L-或D-乳酸盐消失率之间是否存在关联。随着实验时间的推移，每克组织中的脂肪细胞数量减少；然而，与精料育肥的阉牛相比，采食干草的阉牛每克皮下脂肪组织中的细胞数量较少，但细胞体积较大（分别为0.78±0.04和1.20±0.13×10⁻⁶/克）。不过，这些结果可能是由于采食干草的阉牛干物质和可代谢能量摄入量较高（约25%）。屠宰时（460千克）获得的胴体数据表明，与采食干草的阉牛相比，精料育肥的阉牛具有同等数量或更多的脂肪组织。描述血浆中D-或L-乳酸盐消失的特征与用于脂肪酸合成的乳酸盐利用率并非高度相关。体外将乙酸盐用作脂肪酸合成底物的利用率与用于脂肪酸合成的乳酸盐利用率相关（r = 0.64）。
