Whitehurst G B, Beitz D C, Pothoven M A, Ellison W R, Crump M H
J Nutr. 1978 Nov;108(11):1806-11. doi: 10.1093/jn/108.11.1806.
In vitro experiments were conducted to determine the rates of lactate, acetate, and glucose conversions to glycerol, CO2, and fatty acids by subcutaneous adipose tissue of cattle. To study the pathway for lactate utilization as a carbon source for lipogenesis, the effect of each of four substates (lactate, acetate, pyruvate, and glucose) on the rate of conversion of the other three was determined. The rates of lactate conversion to fatty acids and to glycerol were greater than those for acetate and glucose. Acetate, however, was oxidized to CO2 at greater rates than either lactate or glucose. Acetate was used for fatty acid synthesis at greater rates than pyruvate was less than that from lactate but greater than that from glucose. Acetate additions to the incubation media decreased the synthetic rates from lactate, pyruvate, and glucose. Pyruvate decreased rates of fatty acid synthesis from glucose but slightly (not statistically significant) stimulated synthesis from lactate and acetate. Lactate decreased glucose and pyruvate utilization but had no effect upon that of acetate. Our results clearly demonstrated that lactate can be used for fatty acid synthesis as well as for glycerogenesis by bovine adipose tissue.
进行体外实验以测定牛皮下脂肪组织将乳酸、乙酸盐和葡萄糖转化为甘油、二氧化碳和脂肪酸的速率。为了研究乳酸作为脂肪生成碳源的利用途径，确定了四种底物（乳酸、乙酸盐、丙酮酸和葡萄糖）中的每一种对其他三种底物转化率的影响。乳酸转化为脂肪酸和甘油的速率高于乙酸盐和葡萄糖。然而，乙酸盐氧化为二氧化碳的速率高于乳酸或葡萄糖。乙酸盐用于脂肪酸合成的速率高于丙酮酸，低于乳酸但高于葡萄糖。向孵育培养基中添加乙酸盐会降低乳酸、丙酮酸和葡萄糖的合成速率。丙酮酸降低了葡萄糖的脂肪酸合成速率，但略微（无统计学意义）刺激了乳酸和乙酸盐的合成。乳酸降低了葡萄糖和丙酮酸的利用率，但对乙酸盐的利用率没有影响。我们的结果清楚地表明，乳酸可被牛脂肪组织用于脂肪酸合成以及甘油生成。
