Bergqvist Y, Domeij-Nyberg B
J Chromatogr. 1983 Jan 14;272(1):137-48. doi: 10.1016/s0378-4347(00)86110-1.
The amount of chloroquine and desethyl-chloroquine was determined in samples of total blood and in blood cell fractions from three normal subjects after one oral dose of 1000 mg of chloroquine diphosphate. About 70-85% of the total whole blood content of chloroquine and its metabolite desethyl-chloroquine were recovered in blood cells isolated from whole blood, indicating that these compounds have a high cell/plasma concentration ratio. They were mainly present in thrombocytes and granulocytes. A study of 40 patients taking chloroquine regularly as a treatment for rheumatoid arthritis showed significantly higher concentrations of chloroquine and desethyl-chloroquine in serum than in plasma. The concentration of chloroquine was about two times higher in serum than in plasma and for desethyl-chloroquine the concentration was about four times higher in serum than in plasma. These differences may be explained by a release of chloroquine and desethyl-chloroquine from thrombocytes during the coagulation of blood. The practical implication of the results is that the samples taken for chloroquine determination must be clearly identified as serum or plasma.
给三名正常受试者口服1000毫克磷酸氯喹单剂量后，测定了全血样本及血细胞组分中的氯喹和去乙基氯喹含量。从全血中分离出的血细胞中回收了约70 - 85%的氯喹及其代谢产物去乙基氯喹的全血含量，这表明这些化合物具有较高的细胞/血浆浓度比。它们主要存在于血小板和粒细胞中。一项对40例定期服用氯喹治疗类风湿性关节炎患者的研究表明，血清中氯喹和去乙基氯喹的浓度显著高于血浆。氯喹在血清中的浓度约为血浆中的两倍，而去乙基氯喹在血清中的浓度约为血浆中的四倍。这些差异可能是由于血液凝固过程中血小板释放氯喹和去乙基氯喹所致。结果的实际意义在于，用于氯喹测定的样本必须明确标识为血清或血浆。
