Verdier F, Clavier F, Deloron P, Blayo M C
Pathol Biol (Paris). 1984 May;32(5):359-61.
Chloroquine (Cq) and desethyl-chloroquine ( CqM ) levels were measured by HPLC in the blood, plasma, and erythrocytes of 9 healthy subjects under standard prophylactic treatment (100 mg/day for 10 days) and 8 malarial patients given a therapeutic regimen (10 or 25 mg/kg). Chloroquine levels in various fractions of the healthy subjects were as follows: whole blood: 1 265 +/- 598 nmol/l; plasma: 145 +/- 63 nmol/l of whole blood; erythrocytes: 827 +/- 460 nmol/l of whole blood. The CqM /Cq ratio in malarial patients varied from 0.4 to 0.8. These results show that Cq levels and Cq metabolization varied significantly from one individual to the next. Above all, they demonstrate the presence of Cq in other types of blood cells. This underlines the practical importance of the conditions of chloroquine assay in blood.
采用高效液相色谱法（HPLC）测定了9名接受标准预防治疗（100毫克/天，共10天）的健康受试者以及8名接受治疗方案（10或25毫克/千克）的疟疾患者血液、血浆和红细胞中的氯喹（Cq）及去乙基氯喹（CqM）水平。健康受试者各组分中的氯喹水平如下：全血：1265±598纳摩尔/升；血浆：占全血的145±63纳摩尔/升；红细胞：占全血的827±460纳摩尔/升。疟疾患者的CqM/Cq比值在0.4至0.8之间。这些结果表明，氯喹水平和氯喹代谢在个体之间存在显著差异。最重要的是，它们证明了氯喹在其他类型血细胞中的存在。这突出了血液中氯喹检测条件的实际重要性。
