Marzo A, Quadro G, Treffner E
J Chromatogr. 1983 Jan 14;272(1):95-102. doi: 10.1016/s0378-4347(00)86106-x.
Extractive alkylation was carried out on fenquizone, a sulphonamide diuretic, in order to devise a suitable method for its determination in pharmacokinetic and bioavailability studies. After extraction as a tetramethyl derivative, fenquizone was evaluated by gas-liquid chromatography with a 63Ni electron-capture detector, which enables a limit of detection of 2 ng/ml of plasma or urine to be achieved. Linearity was verified in a range of 50- 10,000 pg for each injection with a fenquizone/internal standard ratio ranging from 4:1 to 1:4. Determination is very rapid, as one analysis only takes 5 min. The preliminary results of the pharmacokinetic study performed in a volunteer human subject after a single oral administration of the drug are presented in this paper in terms of the plasma levels and the cumulative urinary excretion.
对磺酰胺类利尿剂芬喹宗进行萃取烷基化，以便设计出一种适用于其药代动力学和生物利用度研究的测定方法。作为四甲基衍生物萃取后，采用配有⁶³Ni电子捕获检测器的气液色谱法对芬喹宗进行评估，该方法可实现血浆或尿液检测限为2 ng/ml。每次进样时，在50 - 10,000 pg范围内验证线性，芬喹宗/内标比为4:1至1:4。测定速度非常快，一次分析仅需5分钟。本文给出了在一名志愿者受试者单次口服该药物后进行的药代动力学研究的初步结果，包括血浆水平和累积尿排泄量。
