Marzo A, Treffner E, Neggiani P P, Staibano G
J Chromatogr. 1984 Sep 14;310(1):51-9. doi: 10.1016/0378-4347(84)80067-5.
The present paper reports the analytical conditions allowing lofemizole, a new non-steroidal anti-inflammatory drug, to be evaluated in biological fluids for pharmacokinetic and bioavailability investigations. The first approach led to an N-methyl derivative of lofemizole which could be successfully analysed by gas--chromatography employing a flame-ionization detector, reaching a sensitivity of 2 micrograms/ml. The second approach led to the N-(2-chlorobenzoyl) derivative of lofemizole which was suitable for pharmacokinetic investigation using gas--liquid chromatography with electron-capture detection, and reaching a much higher sensitivity of 10 ng/ml of plasma. Recovery of the extraction, reproducibility and specificity were all satisfactory with both methods. Since the first method employing flame-ionization detection was suitable for pharmacokinetic investigations in animal species, this paper describes both methods on a comparative basis.
本文报道了可用于在生物流体中评估新型非甾体抗炎药洛非米唑的分析条件，以便进行药代动力学和生物利用度研究。第一种方法得到了洛非米唑的N - 甲基衍生物，该衍生物可通过使用火焰离子化检测器的气相色谱法成功分析，灵敏度达到2微克/毫升。第二种方法得到了洛非米唑的N -（2 - 氯苯甲酰基）衍生物，该衍生物适用于采用电子捕获检测的气液色谱法进行药代动力学研究，血浆灵敏度高达10纳克/毫升。两种方法的提取回收率、重现性和特异性均令人满意。由于采用火焰离子化检测的第一种方法适用于动物物种的药代动力学研究，本文在比较的基础上描述了这两种方法。
