Toti M, Rizzi R, Almi P, Palla M, Bonino F
J Med Virol. 1983;11(2):139-45. doi: 10.1002/jmv.1890110208.
HBsAg bound to IgM was measured in the serum of HBsAg carriers with acute hepatitis using a radioimmunoassay based on selective absorption of IgM on solid phase coated with antiserum to human IgM. HBsAg/IgM was detected in 94 (100%) patients with acute type B hepatitis during the acute phase of infection and persisted after the fourth week only in 13 of them, who developed chronic liver disease. HBsAg/IgM was detected only in 1 patient out of 15 carriers of the HBsAg with superimposed non-B hepatitis. No activity was found in serum of 20 patients with acute HBsAg-negative hepatitis. The nature of the IgM component of the complex is uncertain, however, blocking experiments of the HBsAg/IgM reaction with polymerized human albumin suggest that the IgM component of the complex might represent antibody to the denatured protein. Persistent HBsAg/IgM complex detection in patients with acute type B hepatitis provides a useful tool to predict transition of HBV infection to chronicity. Its absence in patients with acute HBsAg-positive hepatitis is indicative of non-B hepatitis in chronic carriers of the HBsAg.
采用基于人IgM抗血清包被的固相上IgM选择性吸收的放射免疫分析法，检测急性肝炎HBsAg携带者血清中与IgM结合的HBsAg。在94例（100%）急性乙型肝炎患者感染急性期检测到HBsAg/IgM，仅13例在第四周后仍持续存在，这些患者发展为慢性肝病。15例HBsAg携带者合并非乙型肝炎患者中仅1例检测到HBsAg/IgM。20例急性HBsAg阴性肝炎患者血清中未检测到活性。然而，复合物中IgM成分的性质尚不确定，用聚合人白蛋白阻断HBsAg/IgM反应的实验表明，复合物中的IgM成分可能代表对变性蛋白的抗体。急性乙型肝炎患者持续检测到HBsAg/IgM复合物为预测HBV感染向慢性转变提供了有用工具。急性HBsAg阳性肝炎患者未检测到该复合物表明HBsAg慢性携带者患有非乙型肝炎。
