Palla M, Rizzi R, Toti M, Almi P, Rizzetto M, Bonino F, Purcell R
Infect Immun. 1983 Sep;41(3):950-8. doi: 10.1128/iai.41.3.950-958.1983.
Hepatitis B surface antigen (HBsAg) bound to immunoglobulin M (IgM) was detected in sera of HBsAg carriers by a radioimmunoassay based on selective absorption of the immunoglobulin on a solid phase coated with antiserum to human IgM. Isopycnic banding and rate-zonal sedimentation have shown that the reaction is related to particulate forms of the HBsAg complexed with IgM. The binding of IgM possibly occurred because of a selective affinity of these molecules to the surface of HBsAg particles. HBsAg/IgM was found transiently in 24 of 25 (96%) patients with acute self-limited hepatitis B and persistently in 6 of 25 patients whose acute hepatitis B progressed to chronicity. It was also found in 20 of 39 (51%) chronic HBsAg carriers with inactive and asymptomatic infection. The HBsAg/IgM phenomenon is not dependent on replication of hepatitis B virions; its persistence in patients with acute hepatitis B may provide complementary evidence of transition of the infection to chronicity.
通过一种放射免疫测定法，在乙肝表面抗原（HBsAg）携带者的血清中检测到了与免疫球蛋白M（IgM）结合的HBsAg。该测定法基于免疫球蛋白在包被有抗人IgM抗血清的固相上的选择性吸附。等密度离心和速率区带沉降表明，该反应与与IgM复合的HBsAg的颗粒形式有关。IgM的结合可能是由于这些分子对HBsAg颗粒表面的选择性亲和力。在25例急性自限性乙型肝炎患者中有24例（96%）短暂发现HBsAg/IgM，在25例急性乙型肝炎进展为慢性的患者中有6例持续发现。在39例慢性HBsAg携带者中的20例（51%）处于非活动和无症状感染状态的患者中也发现了HBsAg/IgM。HBsAg/IgM现象不依赖于乙肝病毒颗粒的复制；其在急性乙型肝炎患者中的持续存在可能为感染向慢性转变提供补充证据。
