源自大鼠皮质突触体的蛋白脂质体中的高亲和力胆碱摄取和钙依赖性乙酰胆碱释放。

High affinity choline uptake and calcium-dependent acetylcholine release in proteoliposomes derived from rat cortical synaptosomes.

作者信息

Meyer E M, Cooper J R

出版信息

J Neurosci. 1983 May;3(5):987-94. doi: 10.1523/JNEUROSCI.03-05-00987.1983.

DOI:10.1523/JNEUROSCI.03-05-00987.1983

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6564513/

Abstract

Proteoliposomes were prepared from rat cortical synaptic plasma membranes that retained high and low affinity choline transport. High affinity transport was inhibited by hemicholinium-3 in a competitive manner and was apparently dependent on membrane potential or ion gradients. Proteoliposomes supplemented with an acetylcholine-generating system were able to synthesize [3H]ACh de novo from [3H]choline taken up by high or low affinity transport; however, we were able to specifically load proteoliposomes derived from cholinergic membranes by using a low choline concentration. [3H]ACh, but not [3H]choline, was subsequently released in a calcium-dependent manner by K+ depolarization. This preparation offers a simplified membrane system for studying functional high affinity choline transport and nonvesicular ACh release.

摘要

蛋白脂质体是由保留高亲和力和低亲和力胆碱转运的大鼠皮质突触质膜制备而成。高亲和力转运以竞争性方式被半胱氨酸-3抑制，并且显然依赖于膜电位或离子梯度。补充有乙酰胆碱生成系统的蛋白脂质体能够从通过高亲和力或低亲和力转运摄取的[3H]胆碱中重新合成[3H]乙酰胆碱；然而，我们能够通过使用低胆碱浓度特异性地装载源自胆碱能膜的蛋白脂质体。随后，[3H]乙酰胆碱而非[3H]胆碱通过钾离子去极化以钙依赖的方式释放。这种制备方法为研究功能性高亲和力胆碱转运和非囊泡性乙酰胆碱释放提供了一种简化的膜系统。

相似文献

1

High affinity choline uptake and calcium-dependent acetylcholine release in proteoliposomes derived from rat cortical synaptosomes.源自大鼠皮质突触体的蛋白脂质体中的高亲和力胆碱摄取和钙依赖性乙酰胆碱释放。

J Neurosci. 1983 May;3(5):987-94. doi: 10.1523/JNEUROSCI.03-05-00987.1983.

2

High-affinity choline transport in proteoliposomes derived from rat cortical synaptosomes.

Science. 1982 Aug 27;217(4562):843-5. doi: 10.1126/science.7100928.

3

Calcium-dependent [3H]acetylcholine release and muscarinic autoreceptors in rat cortical synaptosomes during development.

Neurochem Res. 1983 May;8(5):621-8. doi: 10.1007/BF00964701.

4

Arachidonic acid inhibits choline uptake and depletes acetylcholine content in rat cerebral cortical synaptosomes.花生四烯酸抑制大鼠大脑皮质突触体对胆碱的摄取并消耗乙酰胆碱含量。

J Neurochem. 1988 Apr;50(4):1309-18. doi: 10.1111/j.1471-4159.1988.tb10609.x.

5

Antibodies directed against ubiquitin inhibit high affinity [3H]choline uptake in rat cerebral cortical synaptosomes.针对泛素的抗体可抑制大鼠大脑皮质突触体中高亲和力的[3H]胆碱摄取。

J Biol Chem. 1986 Nov 5;261(31):14365-8.

6

Inhibition of choline efflux results in enhanced acetylcholine synthesis and release in the guinea-pig corticocerebral synaptosomes.胆碱外流的抑制导致豚鼠大脑皮质突触体中乙酰胆碱合成和释放增加。

Neurochem Int. 1992 Feb;20(2):219-27. doi: 10.1016/0197-0186(92)90171-m.

7

The subsynaptosomal distribution and release of [3H]acetylcholine synthesized by rat cerebral cortical synaptosomes.大鼠大脑皮质突触体合成的[3H]乙酰胆碱在突触小体下的分布及释放

Neurochem Res. 1981 Dec;6(12):1265-82. doi: 10.1007/BF00964348.

8

Effects of 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (AH5183) on rat cortical synaptosome choline uptake, acetylcholine storage and release.4-（2-羟乙基）-1-哌嗪乙磺酸（AH5183）对大鼠皮质突触体胆碱摄取、乙酰胆碱储存和释放的影响。

Brain Res. 1985 Dec 16;359(1-2):208-14. doi: 10.1016/0006-8993(85)91430-1.

9

Effects of dipalmitoylphosphatidylcholine liposomes on high affinity transport of choline and synthesis of acetylcholine in cerebral cortical synaptosomes in the rat.

Neuropharmacology. 1987 May;26(5):453-6. doi: 10.1016/0028-3908(87)90026-8.

10

Dipalmitoylphosphatidylcholine liposomes reduce [3H]acetylcholine levels in an eserine-sensitive manner in rat cerebral cortical synaptosomes.

Neurochem Res. 1989 Feb;14(2):161-7. doi: 10.1007/BF00969633.

引用本文的文献

1

In vitro reconstitution of neurotransmitter release.神经递质释放的体外重建。

Neurochem Res. 1998 May;23(5):709-18. doi: 10.1023/a:1022451224748.

2

Acetylcholine release and the cholinergic genomic locus.乙酰胆碱释放与胆碱能基因组位点。

Mol Neurobiol. 1998 Feb;16(1):1-20. doi: 10.1007/BF02740600.

3

Reconstitution of a functional synaptosomal membrane possessing the protein constituents involved in acetylcholine translocation.重构具有参与乙酰胆碱转运的蛋白质成分的功能性突触体膜。

Proc Natl Acad Sci U S A. 1984 Jan;81(1):277-81. doi: 10.1073/pnas.81.1.277.

4

Kinetics of irreversible inhibition of choline transport in synaptosomes by ethylcholine mustard aziridinium.乙胆碱氮芥吖丙啶对突触体中胆碱转运的不可逆抑制动力学

J Membr Biol. 1984;82(3):259-68. doi: 10.1007/BF01871635.

5

Choline fluxes in synaptosomal membrane vesicles.突触体膜囊泡中的胆碱通量。

Cell Mol Neurobiol. 1985 Sep;5(3):285-96. doi: 10.1007/BF00711013.

6

Reconstitution of carrier-mediated choline transport in proteoliposomes prepared from presynaptic membranes of Torpedo electric organ, and its internal and external ionic requirements.从电鳐电器官突触前膜制备的蛋白脂质体中载体介导的胆碱转运的重建及其内外离子需求。

J Membr Biol. 1985;85(2):111-9. doi: 10.1007/BF01871264.

7

Depolarization-induced release of propranolol and atenolol from rat cortical synaptosomes.去极化诱导大鼠皮质突触体释放普萘洛尔和阿替洛尔。

Br J Pharmacol. 1985 Feb;84(2):499-510. doi: 10.1111/j.1476-5381.1985.tb12934.x.

8

Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.一种介导乙酰胆碱钙依赖性转运的突触前膜蛋白的纯化

Proc Natl Acad Sci U S A. 1986 Dec;83(23):9226-30. doi: 10.1073/pnas.83.23.9226.

9

High-affinity choline transporter in synaptosmal membranes.

J Protein Chem. 1989 Jun;8(3):372-4. doi: 10.1007/BF01674284.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。