突触体膜囊泡中的胆碱通量。
Choline fluxes in synaptosomal membrane vesicles.
作者信息
Breer H, Knipper M
出版信息
Cell Mol Neurobiol. 1985 Sep;5(3):285-96. doi: 10.1007/BF00711013.
Abstract
Synaptic plasma membrane vesicles isolated from the highly cholinergic nervous tissue of insects were used to study the translocation of choline across the membrane via a high-affinity carrier-mediated mechanism energized by ion gradients as the sole driving force. The uphill movement of choline, energized mainly by the Na+ gradient, attained levels of choline severalfold the final equilibrium value at the peak of the overshoot. Efflux of choline required the presence of internal sodium ions and was promoted by external choline if Na+ was present. External choline inhibited choline efflux in the absence of sodium. It is concluded that the efflux of choline is in many aspects symmetrical with its uptake.
摘要
从昆虫高度胆碱能神经组织中分离出的突触质膜囊泡，用于研究胆碱通过高亲和力载体介导机制跨膜转运，该机制由离子梯度作为唯一驱动力提供能量。胆碱的上坡转运主要由Na+梯度提供能量，在过冲峰值时，胆碱水平达到最终平衡值的几倍。胆碱外流需要内部钠离子的存在，并且如果存在Na+，外部胆碱会促进其外流。在没有钠的情况下，外部胆碱会抑制胆碱外流。得出的结论是，胆碱的外流在许多方面与其摄取是对称的。
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本文引用的文献
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