Hongo K, Chiba S
J Pharmacol Methods. 1983 Feb;9(1):83-90. doi: 10.1016/0160-5402(83)90054-2.
A new model of isolated vascular preparation was developed in the dog. Several canine arteries were isolated and suspended in a bath and perfused under a constant flow rate with Ringer-Locke solution. In each experiment, resting perfusion pressure was set at a constant level at 40-60 mm Hg. Drugs were administered in the endothelial side of the artery through a cannulated tubing, and the response was obtained as changes in the perfusion pressure. By inserting the polyethylene cannula into the artery, the space between the luminar wall of the artery and the cannula was decreased enough to obtain a suitable perfusion pressure, while the cannula was filled with the fluid by pumping. Although some other methods have been used to observe contractile responses of the artery, this method might be superior to them because: 1) drugs act only on the luminar wall; 2) vascular responses to drugs, not only constriction, but also dilatation, in nontreated preparations, are readily observed in a relatively larger artery (2-3 mm I.D.); 3) the vascular reactivity is kept in a stable condition over 7-8 hours at 37 degrees C. We used this preparation to examine the contractile responses of the common, internal, external carotid, and femoral arteries to 5-hydroxytryptamine.
在犬类动物中建立了一种新的离体血管制备模型。分离出几条犬动脉，将其悬挂于浴槽中，并用林格 - 洛克溶液以恒定流速进行灌注。在每个实验中，静息灌注压设定在40 - 60 mmHg的恒定水平。通过插管在动脉的内皮侧给药，并以灌注压的变化作为反应。通过将聚乙烯插管插入动脉，动脉腔壁与插管之间的空间减小到足以获得合适的灌注压，同时通过泵注使插管充满液体。虽然已经使用了一些其他方法来观察动脉的收缩反应，但这种方法可能优于它们，原因如下：1）药物仅作用于腔壁；2）在未经处理的制剂中，在相对较大的动脉（内径2 - 3 mm）中可以很容易地观察到血管对药物的反应，不仅有收缩，还有扩张；3）在37℃下，血管反应性在7 - 8小时内保持稳定状态。我们使用这种制备方法来研究颈总动脉、颈内动脉、颈外动脉和股动脉对5 - 羟色胺的收缩反应。
