Chiba S, Ohhashi T, Azuma T
Tohoku J Exp Med. 1978 May;125(1):39-43. doi: 10.1620/tjem.125.39.
Canine carotid arteries were isolated, suspended in a bath and perfused under a constant flow rate with arterial blood led from a support dog. Resting perfusion pressure was set at a constant level over 50 mm Hg. Drugs were administered into the endothelial side of the artery through a cannulated tubing and the response was obtained as pressure changes. This preparation was proved to be superior to usual isolated arterial strips, since its reactivity was kept in a stable condition over 5-10 hours at 37 degrees C. Using this preparation, effects of 5-hydroxytryptamine (5-HT) on the internal and external carotid arteries were investigated in comparison with effects of norepinephrine. In the internal carotid artery, 5-HT caused a much more potent vasoconstriction than norepinephrine. On the other hand, in the external carotid artery, 5-HT caused only slight vasoconstriction, while norepinephrine produced a marked vasoconstriction.
分离犬的颈动脉，将其悬挂于浴槽中，并以恒定流速用来自供血犬的动脉血进行灌注。静息灌注压设定在50毫米汞柱以上的恒定水平。通过插管将药物注入动脉的内皮侧，并以压力变化作为反应。这种制备方法被证明优于通常的离体动脉条，因为在37摄氏度下其反应性可在5至10小时内保持稳定状态。使用这种制备方法，研究了5-羟色胺（5-HT）与去甲肾上腺素相比对颈内动脉和颈外动脉的影响。在颈内动脉中，5-HT引起的血管收缩比去甲肾上腺素强得多。另一方面，在颈外动脉中，5-HT仅引起轻微的血管收缩，而去甲肾上腺素则产生明显的血管收缩。
