Tanaka K, Ohtani S, Shigeno K
Poult Sci. 1983 Mar;62(3):452-8. doi: 10.3382/ps.0620452.
The effect of increasing the energy content of the diet through supplementation of various levels of fat or protein on hepatic lipogenesis and activities of associated enzymes of liver was examined in force-fed growing chicks. Hepatic lipogenesis was significantly decreased as the dietary metabolizable energy level was increased through supplementation of fat or protein. The activity of nicotinamide adenine dinucleotide phosphate-malate dehydrogenase (NADP-MDH) (EC 1.1.1.40) in liver was higher (P less than .01) in chicks fed diets containing the lowest energy level. The activity of citrate cleavage enzyme (EC 4.1.3.8) in liver was significantly depressed as the dietary metabolizable energy level increased through supplementation of fat, whereas increasing the dietary metabolizable energy level through protein supplementation resulted in a significant increase in citrate cleavage enzyme activity in liver. Nonesterified fatty acid concentration in serum was significantly increased as the dietary metabolizable energy level was increased through supplementation of fat.
通过强制喂养生长中的雏鸡，研究了通过补充不同水平的脂肪或蛋白质来提高日粮能量含量对肝脏脂肪生成及肝脏相关酶活性的影响。随着通过补充脂肪或蛋白质使日粮代谢能水平升高，肝脏脂肪生成显著降低。在采食能量水平最低日粮的雏鸡中，肝脏中烟酰胺腺嘌呤二核苷酸磷酸 - 苹果酸脱氢酶（NADP - MDH）（EC 1.1.1.40）的活性较高（P < 0.01）。随着通过补充脂肪使日粮代谢能水平升高，肝脏中柠檬酸裂解酶（EC 4.1.3.8）的活性显著降低，而通过补充蛋白质提高日粮代谢能水平则导致肝脏中柠檬酸裂解酶活性显著增加。随着通过补充脂肪使日粮代谢能水平升高，血清中非酯化脂肪酸浓度显著增加。
