Sobczak E, Kocoń J
Zentralbl Mikrobiol. 1983;138(1):25-37.
The aim of the present study was to determine the structure of bacterial cells from Propionibacterium genus as well as their structure during the cellular division. On the basis of the observations made in the electron transmission microscope, in uranyl-acetates-tained preparations of ultra-thin specimens of bacteria, it was stated that propionic bacteria appeared in a shape of short rods, possessing regular profiles of cell walls as opposed to Gram-negative bacteria with a very creased edge line. Besides, it was observed that division of cells had place by formation of septum, most probably preceded by the division of mezosome, which is a signal for creating the divisional wall. In the conducted studies, the following phenomena were started: presence of membraneous structure of mezosomes, which is linked with the chain of circular DNA in bacterial cell, appearance of numerous ribosomes in the regions of tangled threads of nucleic acids, and existence of other undefinite elements. Mezosome present in the cell of propionic bacteria is probably linked with the cell wall at least in two places and on the surface of external cell wall at the site of its linking; it causes the change in electronic density, demonstrated by the undefined holes or scars in cell wall. This finding gives the possibility of distinguishing this genus of Propionibacterium, in the respect of morphology, from other bacteria what, in the opinion of the authors, is a new achievement in the studies on the structure of propionic bacteria.
本研究的目的是确定丙酸杆菌属细菌细胞的结构及其细胞分裂过程中的结构。基于在电子透射显微镜下对细菌超薄标本的醋酸铀染色制剂的观察，发现丙酸杆菌呈短杆状，细胞壁轮廓规则，这与边缘线非常褶皱的革兰氏阴性菌不同。此外，观察到细胞通过形成隔膜进行分裂，最有可能在间体分裂之前发生，间体分裂是形成分裂壁的信号。在进行的研究中，发现了以下现象：间体的膜状结构的存在，其与细菌细胞中的环状DNA链相连；在核酸缠结区域出现大量核糖体；以及存在其他不确定的成分。丙酸杆菌细胞中的间体可能至少在两个位置与细胞壁相连，并在其连接部位的外部细胞壁表面；它导致电子密度的变化，表现为细胞壁中不确定的孔洞或疤痕。这一发现使得在形态学方面能够将丙酸杆菌属与其他细菌区分开来，作者认为这是丙酸杆菌结构研究中的一项新成果。
