Colorimetric estimation of vitamin B1 in pharmaceutical preparations.
作者信息
Sane R T, Ghorpade U A
出版信息
Acta Vitaminol Enzymol. 1983;5(1):29-34.
PMID:6846104
Abstract
A simple colorimetric method of estimation of thiamine from various pharmaceutical formulations is reported. Thiamine forms a yellow coloured complex with para-aminophenol in ammonia medium. The complex is extracted in chloroform (lambda max. 430 nm). The Beer's law is obeyed from 1.5 to 10 micrograms/ml.
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