Vanderslice J T, Brownlee S G, Maire C E, Reynolds R D, Polansky M
Am J Clin Nutr. 1983 May;37(5):867-71. doi: 10.1093/ajcn/37.5.867.
A previously developed high performance chromatographic method has been modified slightly and used to determine the forms of vitamin B6 in human milk. The chromatographic traces are free of compounds that would interfere with the two principal forms found, pyridoxal and pyridoxal phosphate. The method is fast and reliable; it yields recoveries of from 90 to 106% for the vitamers and agrees with results obtained on the same samples with the standard microbiological assay.
一种先前开发的高效色谱方法已稍作修改,用于测定人乳中维生素B6的形态。色谱图中不存在会干扰所发现的两种主要形态(吡哆醛和磷酸吡哆醛)的化合物。该方法快速可靠;维生素各成分的回收率在90%至106%之间,并且与使用标准微生物测定法对相同样品所得结果一致。