Mullins M M
Am J Physiol. 1983 May;244(5):H652-5. doi: 10.1152/ajpheart.1983.244.5.H652.
To ascertain the effect of aldosterone on body fluid volumes in neonatal, prehypertensive spontaneously hypertensive rats (SHR), we studied these animals at 12 days using age-matched Wistar-Kyoto (WKY) as normotensive controls. Some pups of each strain were treated with spironolactone (1.5 micrograms/g body wt) on days 10-12. Total body water (TBW, by dessication) and extracellular fluid (ECF, Na2 35SO4 space) volumes were significantly larger in SHR than in WKY, whereas plasma volumes (125I-serum albumin space) were not different. Thus the enlarged ECF was due to preferential expansion of the interstitial fluid (ISF) space. Treatment of SHR with spironolactone reduced TBW and ISF to values not different from untreated WKY and also reduced plasma volume to some extent. These results indicate 1) significant ISF volume expansion occurs in SHR prior to elevation of blood pressure, and 2) the previously observed elevation in plasma aldosterone in SHR at this age probably mediates the volume expansion.
为了确定醛固酮对新生期、高血压前期自发性高血压大鼠（SHR）体液容量的影响，我们在12日龄时对这些动物进行了研究，使用年龄匹配的Wistar-Kyoto（WKY）大鼠作为正常血压对照。在第10至12天，对每个品系的一些幼崽用螺内酯（1.5微克/克体重）进行处理。SHR的总体水（TBW，通过干燥法测定）和细胞外液（ECF，Na2 35SO4空间）容量显著大于WKY，而血浆容量（125I-血清白蛋白空间）无差异。因此，ECF的增大是由于间质液（ISF）空间的优先扩张。用螺内酯处理SHR可使TBW和ISF降低至与未处理的WKY无差异的值，并且在一定程度上也降低了血浆容量。这些结果表明：1）在血压升高之前，SHR中发生了显著的ISF容量扩张；2）此前观察到的该年龄SHR血浆醛固酮升高可能介导了容量扩张。
