Barajas L, Wang P
Anat Rec. 1983 Feb;205(2):185-95. doi: 10.1002/ar.1092050209.
In this investigation we have combined the methods of ultrastructural demonstration of acetylcholinesterase activity with electron microscopic autoradiography for the demonstration of norepinephrine uptake. The results show electron-dense deposits indicative of acetylcholinesterase activity associated with perivascular axons overlaid by concentrations of silver grains representing exogenous tritiated norepinephrine. Forty-five percent of the intervaricose regions and 19% of the varicosities overlaid by autoradiographic grains showed "moderate" amounts of cholinesterase staining. A greater proportion of autoradiographic grains was observed on the varicosities than in the intervaricose regions; however, the amount of acetylcholinesterase activity was greater in the intervaricose regions than in the varicosities. This investigation provides evidence for the presence of periaxonal acetylcholinesterase staining in adrenergic axons in the rat kidney.
在本研究中，我们将乙酰胆碱酯酶活性的超微结构示踪方法与用于去甲肾上腺素摄取示踪的电子显微镜放射自显影法相结合。结果显示，电子致密沉积物表明乙酰胆碱酯酶活性与血管周围轴突相关，这些轴突上覆盖着代表外源性氚标记去甲肾上腺素的银粒聚集。放射自显影片覆盖的曲张间隙区域的45%和曲张体的19%显示出“中等”量的胆碱酯酶染色。在曲张体上观察到的放射自显影颗粒比例高于曲张间隙区域；然而，曲张间隙区域的乙酰胆碱酯酶活性量大于曲张体。本研究为大鼠肾脏肾上腺素能轴突中轴突周围乙酰胆碱酯酶染色的存在提供了证据。
