Chalon J, Hillman D, Gross S, Eisner M, Tang C K, Turndorf H
Anesth Analg. 1983 Jun;62(6):565-7.
The effect of halothane on prenatal development was assessed using the appearance of postnatal tolerance to the anesthetic and its effect on brain weight. Eighteen 3-month-old mice were repeatedly tested in a rotating cage for loss of righting reflex during exposure to increasing concentrations of halothane on 15 occasions to determine whether or not tolerance to halothane developed. Of these, nine mice were born to dams exposed for 30 min to 2% halothane on days 14 and 17 of gestation. The other nine mice (controls) were born to dams exposed to 100% oxygen for 30 min at the same stage of pregnancy. There was no significant difference in tolerance to halothane between the groups during the first nine days of repeated exposure to halothane. By the 13th and 15th days, however, mice exposed to halothane in utero became more tolerant to 1% halothane than did controls (P less than 0.025). In addition, the mean total brain weight of six 7-week-old mice exposed to 2% halothane in utero for 30 min on days 14 and 17 of gestation was found to be significantly less than the mean total brain weight of six control mice not exposed to halothane in utero (20.83 +/- 0.27 g and 23.07 +/- 0.51 g, respectively, P less than 0.0025). This difference occurred mainly in the brain stem rather than in the forebrain and cerebellum.
通过评估出生后对麻醉剂的耐受性及其对脑重量的影响，来确定氟烷对产前发育的作用。18只3个月大的小鼠在旋转笼中反复接受测试，在15次实验中，随着氟烷浓度的增加，观察其翻正反射消失的情况，以确定是否会产生对氟烷的耐受性。其中，9只小鼠的母亲在妊娠第14天和第17天暴露于2%氟烷中30分钟。另外9只小鼠（对照组）的母亲在相同孕期阶段暴露于100%氧气中30分钟。在反复接触氟烷的前九天，两组小鼠对氟烷的耐受性没有显著差异。然而，到第13天和第15天，子宫内接触氟烷的小鼠比对照组对1%氟烷的耐受性更强（P小于0.025）。此外，发现6只在妊娠第14天和第17天子宫内暴露于2%氟烷中30分钟的7周龄小鼠的平均全脑重量显著低于6只未在子宫内接触氟烷的对照小鼠的平均全脑重量（分别为20.83±0.27克和23.07±0.51克，P小于0.0025）。这种差异主要发生在脑干，而非前脑和小脑。
