Nicolini C
Anticancer Res. 1983 Mar-Apr;3(2):63-86.
Characterization of native nuclei by new unharmful biophysical methods, coupled both with the determination of lower order structures in DNA and isolated chromatin, by a variety of sophisticated physicochemical probes and with the intranuclear DNA distribution monitored in situ by high resolution image analysis, recently, point to a unique organization of chromatin-DNA from the secondary up to the quinternary level. Based on the wide range of experimental findings, hereby reviewed in details, a three-dimensional model for DNA structure is proposed from the nuclear level down to the genes, functional units hereby associated with specific structural repeating units. This "fibrosome" model not only accounts for many of the independent observations on the physical and chemical properties of chromatin-DNA, and on the nuclear scaffold and pores, but also accounts for most recent, yet unexplained, discoveries on eukaryotic gene structure, mapping and coding, suggesting a mechanism by which gene expression and cell function may be controlled.
通过新的无害生物物理方法对天然细胞核进行表征，同时结合使用各种精密的物理化学探针来测定DNA和分离染色质中的低阶结构，并通过高分辨率图像分析对核内DNA分布进行原位监测，最近的研究指出了从二级到五级水平染色质-DNA的独特组织形式。基于广泛的实验结果（在此详细综述），提出了一个从核水平到基因的DNA结构三维模型，其中功能单元与特定的结构重复单元相关联。这种“纤维体”模型不仅解释了关于染色质-DNA的物理和化学性质、核支架和核孔的许多独立观察结果，还解释了关于真核基因结构、图谱绘制和编码的最新但尚未解释的发现，暗示了一种控制基因表达和细胞功能的机制。
