金黄色葡萄球菌肠毒素B生产与纯化的改良方法。
Modified method for production and purification of Staphylococcus aureus enterotoxin B.
作者信息
Melconian A K, Flandrois J P, Fleurette J
出版信息
Appl Environ Microbiol. 1983 Mar;45(3):1140-3. doi: 10.1128/aem.45.3.1140-1143.1983.
Abstract
A medium containing 4% bio-trypcase and 1% yeast extract was used for the production of Staphylococcus aureus enterotoxin B. The yield obtained was estimated at 200 micrograms of enterotoxin per ml of S. aureus S-6 culture supernatant. The purification method involves chromatography on Biorex 70 resin, isoelectric focusing, and gel filtration on Sephadex G-100. The purified enterotoxin (isoionic point, pH 8.55) was shown to be homogenous protein with a molecular weight of 29,000 when tested by gel electrophoresis.
摘要
一种含有4%生物胰蛋白酶和1%酵母提取物的培养基被用于生产金黄色葡萄球菌肠毒素B。所获得的产量估计为每毫升金黄色葡萄球菌S-6培养上清液中有200微克肠毒素。纯化方法包括在Biorex 70树脂上进行色谱分离、等电聚焦以及在Sephadex G-100上进行凝胶过滤。经凝胶电泳测试，纯化后的肠毒素（等离子点，pH 8.55）显示为分子量为29,000的纯蛋白。
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