Herbison G J, Jaweed M M, Ditunno J F
Arch Phys Med Rehabil. 1983 May;64(5):217-20.
Juxtamuscular electrodes were implanted unilaterally in six groups of adult female Wistar rats to evaluate the effect of chronic electrical stimulation (ES) during denervation and reinnervation of the rat soleus muscle. Two weeks later, the animals underwent bilateral crush-denervation of the sciatic nerve at the sciatic notch. Six additional groups served as normal controls. The soleus muscles in the crush denervated and control groups were stimulated unilaterally with a 4mA, 4msec duration current given at 10Hz continuously 8 hours each day for 5, 10, 15, 20, 25 or 30 days. At the end of each period, the soleus muscles were evaluated for the muscle weights, acetylcholine (ACh) sensitivity and fibrillation potentials (FPs). The normal muscles were unaffected by the stimulation. The denervated-stimulated soleus muscles were heavier at 10 days (p less than 0.05) and had fewer FPs after 5 to 15 days (p less than 0.01) of electrical stimulation than their matched denervated controls. The ACh sensitivity throughout the experimental period and the fibrillatory activity from 20 to 30 days post-crush were similar in the denervated and the denervated-stimulated muscles. In conclusion, ES reduced the degree of atrophy and the number of fibrillations of the soleus muscle in the denervation stage. However, it neither enhanced nor impaired the reinnervation of muscle.
将近肌电极单侧植入六组成年雌性Wistar大鼠体内，以评估慢性电刺激（ES）在大鼠比目鱼肌去神经和再神经支配过程中的作用。两周后，动物在坐骨切迹处接受双侧坐骨神经挤压去神经处理。另外六组作为正常对照。对挤压去神经组和对照组的比目鱼肌进行单侧刺激，每天持续8小时，以10Hz的频率给予4mA、持续4毫秒的电流，持续5、10、15、20、25或30天。在每个时间段结束时，评估比目鱼肌的肌肉重量、乙酰胆碱（ACh）敏感性和纤颤电位（FPs）。正常肌肉不受刺激影响。与匹配的去神经对照组相比，去神经刺激的比目鱼肌在电刺激10天时更重（p<0.05），在电刺激5至15天后纤颤电位更少（p<0.01）。在整个实验期间，去神经和去神经刺激肌肉的ACh敏感性以及挤压后20至30天的纤颤活动相似。总之，ES在去神经阶段降低了比目鱼肌的萎缩程度和纤颤数量。然而，它既没有增强也没有损害肌肉的再神经支配。
