Kodama H, Matsumoto M, Fuquay J I, Syuto B
Avian Dis. 1983 Jan-Mar;27(1):283-91.
Soluble fractions of Pasteurella multocida strain P1059 were extracted from a single source by four methods, and their immunogenicity was evaluated by challenge exposure in turkeys. The fractions were extracted by 1) heating in 2.5% NaCl, 2) 0.5M potassium thiocyanate, 3) 1.0M sodium salicylate, and 4) prolonged stirring in formalin solution followed by pelleting (LPS-protein antigen). Eighty percent to 90% of infected turkeys were protected in two trials by vaccination with the saline extract or LPS-protein antigen, whereas less consistent protection was associated with the other two preparations. Endotoxin content was the highest in LPS-protein antigen, followed by KSCN, Na salicylate, and saline extract in that order. The four fractions contained at least one common antigen, which had previously been shown to be a surface-protective antigen.
多杀性巴氏杆菌P1059菌株的可溶性组分通过四种方法从单一来源提取，并通过在火鸡中进行攻毒暴露来评估其免疫原性。这些组分的提取方法如下：1）在2.5%氯化钠中加热；2）0.5M硫氰酸钾；3）1.0M水杨酸钠；4）在福尔马林溶液中长时间搅拌，然后离心沉淀（脂多糖-蛋白质抗原）。在两项试验中，用盐水提取物或脂多糖-蛋白质抗原进行疫苗接种，80%至90%的感染火鸡得到了保护，而其他两种制剂的保护效果则不太一致。脂多糖-蛋白质抗原中的内毒素含量最高，其次依次是硫氰酸钾、水杨酸钠和盐水提取物。这四种组分至少含有一种共同抗原，该抗原先前已被证明是一种表面保护性抗原。
