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一种通过荧光偏振研究蛋白质介导的脂质交换的简单测定方法。

A simple assay to study protein-mediated lipid exchange by fluorescence polarization.

作者信息

Xü Y H, Gietzen K, Galla H J, Sackmann E

出版信息

Biochem J. 1983 Jan 1;209(1):257-60. doi: 10.1042/bj2090257.

DOI:10.1042/bj2090257
PMID:6847612
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154080/
Abstract

We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic-phase-transition curves were taken after mixing two vesicle preparations of lipids exhibiting different gel-to-liquid phase transitions. From the heights of each phase-transition step we were able to follow the lipid transfer process without separating the two vesicle preparations.

摘要

我们以二苯基己三烯作为光学探针，通过荧光偏振测量研究了小囊泡之间蛋白质介导的磷脂转移。在混合两种具有不同凝胶态到液晶态转变的脂质囊泡制剂后，绘制了热致相变曲线。从每个相变步骤的高度，我们能够追踪脂质转移过程，而无需分离这两种囊泡制剂。

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本文引用的文献

1
Protein-catalyzed phospholipid exchange between gel and liquid-crystalline phospholipid vesicles.蛋白质催化的凝胶与液晶磷脂囊泡之间的磷脂交换。
Biochemistry. 1981 Jan 6;20(1):146-51. doi: 10.1021/bi00504a024.
2
Two nonspecific phospholipid exchange proteins from beef liver. 2. Use in studying the asymmetry and transbilayer movement of phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin in intact rat erythrocytes.来自牛肝的两种非特异性磷脂交换蛋白。2. 用于研究完整大鼠红细胞中磷脂酰胆碱、磷脂酰乙醇胺和鞘磷脂的不对称性及跨膜运动。
Biochemistry. 1980 Apr 1;19(7):1440-7. doi: 10.1021/bi00548a027.
3
Participation of soluble liver proteins in the exchange of membrane phospholipids.可溶性肝蛋白参与膜磷脂的交换。
Biochim Biophys Acta. 1969 Oct 14;193(1):105-16. doi: 10.1016/0005-2736(69)90063-7.
4
Phosphatidyl-ethanolamine: differential labelling in intact cells and cell ghosts of human erythrocytes by a membrane-impermeable reagent.磷脂酰乙醇胺：一种膜不可渗透试剂对人红细胞完整细胞和细胞空壳的差异标记
J Mol Biol. 1972 Nov 28;71(3):523-8. doi: 10.1016/s0022-2836(72)80020-2.
5
Transfer of phospholipids between membranes.膜间磷脂的转移。
Biochim Biophys Acta. 1974 Sep 16;344(2):95-117. doi: 10.1016/0304-4157(74)90001-x.
6
Phosphatidylcholine exchange protein from beef liver.来自牛肝的磷脂酰胆碱交换蛋白。
Methods Enzymol. 1974;32:140-6. doi: 10.1016/0076-6879(74)32017-4.
7
Transfer of phosphatidylcholine between liposomes.
Eur J Biochem. 1974 Sep 16;47(3):601-5. doi: 10.1111/j.1432-1033.1974.tb03731.x.
8
The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy.人红细胞膜中磷脂的不对称分布。一项使用磷脂酶和冷冻蚀刻电子显微镜的联合研究。
Biochim Biophys Acta. 1973 Oct 11;323(2):178-93. doi: 10.1016/0005-2736(73)90143-0.
9
Use of Forssman antigen in the study of phosphatidylcholine exchange between liposomes.
Biochim Biophys Acta. 1972 Aug 9;274(2):652-7. doi: 10.1016/0005-2736(72)90215-5.
10
The protein-mediated transfer of phosphatidylcholine between membranes. The effect of membrane lipid composition and ionic composition of the medium.蛋白质介导的磷脂酰胆碱在膜之间的转移。膜脂质组成和介质离子组成的影响。
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