Skinner M K, Griswold M D
Biochem J. 1983 Jan 1;209(1):281-4. doi: 10.1042/bj2090281.
A fluorographic procedure was optimized which utilized acetic acid as the solvent for 2,5-diphenyloxazole (PPO). This procedure was then compared with existing fluorographic procedures which utilize PPO in dimethyl sulphoxide or sodium salicylate in water, and a commercially available fluorographic solution. En3Hance (New England Nuclear Corp.). A comparison of the four methods revealed that all of the procedures resulted in essentially the same efficiency of radioactivity detection. The acetic acid/PPO procedure was found to have several technical advantages. There is no need to pre-fix proteins in gels, and either agarose or acrylamide gels can be used. The acetic acid/PPO procedure was found to be a simple, sensitive and efficient alternative fluorographic method.
优化了一种荧光显影方法，该方法使用乙酸作为2,5-二苯基恶唑（PPO）的溶剂。然后将该方法与现有的荧光显影方法进行比较，现有方法分别是在二甲基亚砜中使用PPO或在水中使用水杨酸钠，以及一种市售的荧光显影溶液En3Hance（新英格兰核公司）。对这四种方法的比较表明，所有方法在放射性检测效率上基本相同。发现乙酸/PPO方法具有几个技术优势。无需预先固定凝胶中的蛋白质，琼脂糖凝胶或丙烯酰胺凝胶均可使用。乙酸/PPO方法是一种简单、灵敏且高效的荧光显影替代方法。
