Ludlow J W, Yuen K C, Consigli R A
Anal Biochem. 1985 Feb 15;145(1):212-5. doi: 10.1016/0003-2697(85)90350-1.
A fluorographic procedure for the detection of [3H]thymidine-labeled deoxyribonucleic acids electrophoresed in agarose gels was developed. 2,5-Diphenyloxazole (PPO) was added to the agarose solution before pouring of the gel for electrophoresis. This procedure did not interfere with the electrophoretic mobility of the DNA molecules. The radioactive detection efficiency was found to be improved over an existing procedure whereby the agarose gel was infused with PPO after electrophoresis with the aid of acetic acid.
开发了一种用于检测在琼脂糖凝胶中电泳的[3H]胸苷标记的脱氧核糖核酸的荧光成像程序。在浇注用于电泳的凝胶之前，将2,5-二苯基恶唑（PPO）添加到琼脂糖溶液中。该程序不会干扰DNA分子的电泳迁移率。发现与现有程序相比，放射性检测效率有所提高，现有程序是在电泳后借助乙酸将PPO注入琼脂糖凝胶中。
