耐辐射球菌DNA中交联导致的突变诱导

Mutation induction by cross-links in DNA of Deinococcus radiodurans.

作者信息

Yatagai F, Kitayama S

出版信息

Biochem Biophys Res Commun. 1983 Apr 29;112(2):458-63. doi: 10.1016/0006-291x(83)91486-9.

Abstract

Reversion of adenine auxotrophic strains of D. radiodurans by cross-links in DNA was studied. Mutation was induced in a wild-type but not in a mitomycin C sensitive mutant after irradiation with near UV (approximately 365nm) in the presence of 4,5',8-trimethylpsoralen (TMP). To get information on implication of cross-links in the observed mutation, cells containing monoadducts in DNA were exposed to near UV in the absence of TMP. The mutation frequency of the wild-type increased with increasing the cross-links in DNA. These results suggest that structural defects after removal of cross-links in DNA induce mutation in D. radiodurans.

摘要

研究了DNA交联对耐辐射异常球菌腺嘌呤营养缺陷型菌株回复突变的影响。在4,5',8-三甲基补骨脂素（TMP）存在的情况下，用近紫外光（约365nm）照射后，野生型菌株发生了突变，但丝裂霉素C敏感突变体未发生突变。为了了解交联在观察到的突变中的作用，将DNA中含有单加合物的细胞在无TMP的情况下暴露于近紫外光。野生型的突变频率随着DNA中交联的增加而增加。这些结果表明，DNA交联去除后的结构缺陷会诱导耐辐射异常球菌发生突变。