耐辐射球菌中的DNA双链断裂及交联去除
DNA double-strand breakage and removal of cross-links in Deinococcus radiodurans.
作者信息
Kitayama S, Asaka S, Totsuka K
出版信息
J Bacteriol. 1983 Sep;155(3):1200-7. doi: 10.1128/jb.155.3.1200-1207.1983.
Abstract
Mitomycin C-sensitive mutants of Deinococcus radiodurans were isolated which were either resistant to or only moderately sensitive to far UV (254 nm) or gamma rays. They were also sensitive to irradiation at 365 nm in the presence of 4,5',8-trimethylpsoralen. They were classified into seven complementary groups (mtcA through mtcG) by transformation experiments. Interstrand cross-links in the DNA duplex induced by mitomycin C were removed in the cells of two mutants (mtcD and mtcE) as in the wild type, whereas the other mutants were deficient in this repair. After a sublethal dosage of mitomycin C, single- and double-stranded cuts of cross-linked DNA were observed in the wild-type cells during postincubation. This removal of cross-links in DNA seems to be indispensable for the cells since their colony-forming ability was markedly reduced if they were postincubated under an inhibitory condition for repair of these lesions.
摘要
分离出了耐放射异常球菌的丝裂霉素C敏感突变体，这些突变体对远紫外线（254nm）或γ射线具有抗性或仅具有中等敏感性。它们在存在4,5',8-三甲基补骨脂素的情况下对365nm的辐射也敏感。通过转化实验将它们分为七个互补组（mtcA至mtcG）。与野生型一样，丝裂霉素C诱导的DNA双链中的链间交联在两个突变体（mtcD和mtcE）的细胞中被去除，而其他突变体在这种修复中存在缺陷。在亚致死剂量的丝裂霉素C处理后，在孵育后观察到野生型细胞中交联DNA的单链和双链切割。DNA中交联的这种去除似乎对细胞是必不可少的，因为如果它们在抑制这些损伤修复的条件下进行孵育后，其集落形成能力会明显降低。
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