Klicka J K, Li S A, Li J J
Biochem Pharmacol. 1983 Feb 1;32(3):497-501. doi: 10.1016/0006-2952(83)90529-4.
Only very low levels of peroxidase activity were detected in castrated male hamster kidneys [1.0 +/- 0.8(S.E.) units/g protein], and chronic estrogen administration, either diethylstilbestrol (DES) or 17 beta-estradiol, for 1-5 months did not result in any appreciable increase in this activity. In contrast, hamster liver peroxidase activity was initially 10- to 20-fold higher than kidney levels, and chronic estrogen treatment for similar periods resulted in up to a 9-fold elevation in the activity of this enzyme. Moreover, the level of liver peroxidase activity in both intact and in 5 alpha-dihydrotestosterone-treated castrate hamsters was 2-fold higher than castrate-untreated values. Pure renal carcinoma induced after 9 months of estrogen treatment exhibited peroxidase values similar to those found in hamster livers [124 +/- 27 (S.E.) units/g protein] following estrogen treatment. When administered concomitantly with DES, tamoxifen significantly reduced the elevated levels of liver peroxidase activity observed after 2 months of DES treatment alone. A high affinity (KA = 0.10 X 10(9)M-1) estrogen receptor was found in liver cytosols of DES-treated hamsters which had increased slightly from untreated castrate levels.
在去势雄性仓鼠肾脏中仅检测到极低水平的过氧化物酶活性[1.0±0.8（标准误）单位/克蛋白质]，并且连续1至5个月给予己烯雌酚（DES）或17β-雌二醇等慢性雌激素处理并未导致该活性有任何明显增加。相比之下，仓鼠肝脏过氧化物酶活性最初比肾脏水平高10至20倍，类似时间段的慢性雌激素处理导致该酶活性升高达9倍。此外，完整仓鼠以及经5α-双氢睾酮处理的去势仓鼠的肝脏过氧化物酶活性水平比未处理的去势仓鼠的值高2倍。雌激素处理9个月后诱导产生的纯肾癌表现出的过氧化物酶值类似于雌激素处理后在仓鼠肝脏中发现的值[124±27（标准误）单位/克蛋白质]。当与DES同时给药时，他莫昔芬显著降低了仅用DES处理2个月后观察到的肝脏过氧化物酶活性升高水平。在经DES处理的仓鼠肝脏胞质溶胶中发现了一种高亲和力（KA = 0.10×10⁹M⁻¹）的雌激素受体，其水平比未处理的去势仓鼠略有增加。
