Callahan G N, Pardi D, Giedlin M A, Allison J P, Morizot D M, Martin W J
J Immunol. 1983 Jan;130(1):471-9.
LT-85 is an alveolegenic adenocarcinoma induced in mutant C3HfB/HeN (C3Hf) mice. This tumor, however, grows preferentially in allogeneic, wild-type C3H/HeN (C3H) mice. The tumor-associated transplantation antigen has been mapped to the K end of the major histocompatibility complex. H-2K antigens were isolated from detergent extracts of LT-85 cells by immunoprecipitation with monoclonal antibody. The tryptic peptides of these antigens were compared, by using high-pressure liquid chromatography, with the tryptic peptides of H-2K antigens isolated from syngeneic mutant C3Hf and ancestral wild-type C3H spleen cells. We found that the H-2K antigens of the LT-85 tumor cells were very similar to, but distinct from, those present on syngeneic C3Hf lymphoid cells. We also found, however, that the H-2K antigens of LT-85 tumor cells were clearly different from the H-2K antigens of allogeneic C3H spleen cells. The H-2K antigens of LT-85 cells are therefore foreign to syngeneic C3Hf cells, but do not represent expression by the tumor cells of the allogeneic H-2K antigens expressed by normal C3H cells. Furthermore, the nature of the differences observed between the H-2K antigens of LT-85 cells and C3Hf and C3H spleen cells strongly suggests that the structure of the H-2K molecule of LT-85 cells is identical in some regions to the H-2K molecule of C3Hf cells, and in other regions to the H-2K molecule of C3H cells.
LT - 85是在突变的C3HfB/HeN（C3Hf）小鼠中诱发的肺泡源性腺癌。然而，这种肿瘤在同种异体的野生型C3H/HeN（C3H）小鼠中优先生长。肿瘤相关移植抗原已被定位到主要组织相容性复合体的K端。通过用单克隆抗体免疫沉淀从LT - 85细胞的去污剂提取物中分离出H - 2K抗原。使用高压液相色谱法，将这些抗原的胰蛋白酶肽与从同基因突变体C3Hf和原始野生型C3H脾细胞中分离出的H - 2K抗原的胰蛋白酶肽进行比较。我们发现LT - 85肿瘤细胞的H - 2K抗原与同基因C3Hf淋巴细胞上的抗原非常相似，但又有所不同。然而，我们还发现LT - 85肿瘤细胞的H - 2K抗原与同种异体C3H脾细胞的H - 2K抗原明显不同。因此，LT - 85细胞的H - 2K抗原对于同基因C3Hf细胞来说是外来的，但并不代表肿瘤细胞表达正常C3H细胞所表达的同种异体H - 2K抗原。此外，LT - 85细胞与C3Hf和C3H脾细胞的H - 2K抗原之间观察到的差异性质强烈表明，LT - 85细胞的H -
