Mackillop W J, Trent J M, Stewart S S, Buick R N
Cancer Res. 1983 Feb;43(2):874-8.
Seven consecutive ascitic tumors were obtained over a 9-month period from a patient with serous adenocarcinoma of the ovary. The tumor cell populations were analyzed for cellular proliferation (labeling index, agar clonogenicity, and self-renewal capacity), for cell differentiation (cell surface expression of carcinoembryonic antigen and histochemical stain for fat accumulation), and for karyotypic changes. Evidence is presented of increased aggressiveness of proliferative features together with a decreasing proportion of cells with differentiated features. Parallel temporal changes were documented in density-volume characteristics of the tumor cell population, from small, high-density to large, low-density cells. The only karyotypic change identified over this period was the loss of one X-chromosome and the increased frequency of cells containing double minute bodies. The progressive characteristics described in this human tumor are not, therefore, associated with gross chromosomal changes. The accumulation of double minute chromosome bodies may be associated with a low-dose methotrexate exposure or with the tumor progression.
在9个月的时间里，从一名卵巢浆液性腺癌患者身上获取了7个连续的腹水肿瘤。对肿瘤细胞群体进行了细胞增殖分析（标记指数、琼脂克隆形成能力和自我更新能力）、细胞分化分析（癌胚抗原的细胞表面表达和脂肪积累的组织化学染色）以及核型变化分析。结果表明，增殖特征的侵袭性增加，同时具有分化特征的细胞比例下降。肿瘤细胞群体的密度-体积特征也有平行的时间变化，从小的、高密度细胞变为大的、低密度细胞。在此期间唯一确定的核型变化是一条X染色体的丢失以及含有双微体的细胞频率增加。因此，这种人类肿瘤中描述的渐进性特征与明显的染色体变化无关。双微染色体体的积累可能与低剂量甲氨蝶呤暴露或肿瘤进展有关。
