Friedlander M L, Taylor I W, Russell P, Tattersall M H
Br J Cancer. 1984 Feb;49(2):173-9. doi: 10.1038/bjc.1984.29.
Detailed flow cytometric analysis of cellular DNA content was performed on neoplastic tissue from 33 patients with malignant common epithelial ovarian tumours in order to investigate the intratumoral stability of ploidy and proliferative fraction. There was a remarkable stability, both spatial and temporal, in the DNA pattern for any particular tumour. Of 24 tumours that were analysed in multiple areas tumour ploidy was found to be a stable marker in all but 3 cases where regional variations were evident. In 9 patients serial analyses were performed on tumour obtained either at initial diagnosis (6 patients) or second look laparotomy (3 patients) and then some time later (7-17 months) at relapse or death and in all cases the tumour ploidy remained unchanged. In addition, 10 ovarian carcinomas established in nude mice have maintained a DNA content during serial passage similar to that of the original implanted tumour. In contrast in 50% of tumours that were evaluable for S-phase analysis we demonstrated a considerable intratumoral variability in the S-phase fraction. We conclude that cellular DNA content is a stable feature of ovarian carcinoma while S-phase fraction is commonly subject to intratumoral variation.
为了研究恶性常见上皮性卵巢肿瘤的肿瘤内倍体稳定性和增殖分数，对33例恶性常见上皮性卵巢肿瘤患者的肿瘤组织进行了细胞DNA含量的详细流式细胞术分析。对于任何特定肿瘤，DNA模式在空间和时间上都具有显著的稳定性。在对多个区域进行分析的24个肿瘤中，除3例有明显区域差异的病例外，其余所有病例的肿瘤倍体均为稳定标记。对9例患者在初次诊断时（6例患者）或二次探查剖腹术时（3例患者）获取的肿瘤进行了系列分析，然后在复发或死亡时（7 - 17个月后）进行了分析，所有病例中肿瘤倍体均保持不变。此外，在裸鼠中建立的10例卵巢癌在连续传代过程中保持了与原始植入肿瘤相似的DNA含量。相比之下，在可进行S期分析的肿瘤中，50%的肿瘤显示出S期分数存在相当大的肿瘤内变异性。我们得出结论，细胞DNA含量是卵巢癌的一个稳定特征，而S期分数通常会受到肿瘤内变异的影响。
