采用同源放射免疫分析法对绵羊胎盘催乳素分泌进行的研究。

Studies on ovine placental lactogen secretion by homologous radioimmunoassay.

作者信息

Handwerger S, Crenshaw C, Maurer W F, Barrett J, Hurley T W, Golander A, Fellows R E

出版信息

J Endocrinol. 1977 Jan;72(1):27-34. doi: 10.1677/joe.0.0720027.

DOI:10.1677/joe.0.0720027

Abstract

The concentrations of ovine placental lactogen (oPL) have been determined in maternal plasma, umbilical cord plasma, and allantoic fluid by an homologous radioimmunoassay for oPL which is sensitive to 0-1 ng hormone. Ovine placental lactogen was first detected in maternal plasma at 41-50 days of gestation and reached a peak concentration of 2547 +/- 226 (S.E.M.) ng/ml at 121-130 days in ewes with singleton gestations. The oPL concentration in cord plasma was 336-4 +/- 60-3 ng/ml and in allantoic fluid was 29-6 +/- 6-4 ng/ml. After surgical removal of the placenta, oPL disappeared from maternal plasma with a half-life of 29-1 +/- 1-3 min.

摘要

采用对0 - 1 ng激素敏感的羊胎盘催乳素（oPL）同源放射免疫分析法，测定了母羊血浆、脐血血浆和尿囊液中oPL的浓度。在妊娠41 - 50天时首次在母羊血浆中检测到羊胎盘催乳素，单胎妊娠母羊在妊娠121 - 130天时达到峰值浓度2547±226（标准误）ng/ml。脐血血浆中oPL浓度为336 - 4±60 - 3 ng/ml，尿囊液中为29 - 6±6 - 4 ng/ml。手术切除胎盘后，oPL从母羊血浆中消失，半衰期为29 - 1±1 - 3分钟。