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蛋白酶抑制剂对小鼠自然杀伤细胞活性的体外作用。

In vitro effects of protease inhibitors on murine natural killer cell activity.

作者信息

Ristow S S, Starkey J R, Hass G M

出版信息

Immunology. 1983 Jan;48(1):1-8.

PMID:6848446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1454011/
Abstract

To test whether proteolytic events are involved in natural killer (NK) cell mediated lysis of tumour cells, twenty-three different protease inhibitors were added to in vitro assays of natural killer cell reactivity. Of all of the materials tested, only tosyl-L-lysine chloromethyl ketone (TLCK), tosyl-L-phenylalanine chloromethyl ketone (TPCK) and benzamidine unequivocally inhibited killing at concentrations approaching those needed to affect appropriate purified proteases. All of the effective inhibitors, and none of the others tested, inhibited binding of effector to target cells. The action of TLCK was focused on both effector and target cells, in that cytolysis was completely inhibited by a 1 hr pretreatment of effectors with 10(-4) M TLCK, and 60% inhibited by a 1 hr treatment of targets only.

摘要

为了检测蛋白水解事件是否参与自然杀伤（NK）细胞介导的肿瘤细胞裂解过程，将23种不同的蛋白酶抑制剂添加到自然杀伤细胞反应性的体外检测中。在所有测试的物质中，只有甲苯磺酰-L-赖氨酸氯甲基酮（TLCK）、甲苯磺酰-L-苯丙氨酸氯甲基酮（TPCK）和苯甲脒在接近影响适当纯化蛋白酶所需的浓度时明确抑制杀伤作用。所有有效的抑制剂，而不是其他测试的抑制剂，均抑制效应细胞与靶细胞的结合。TLCK的作用集中在效应细胞和靶细胞上，因为用10^(-4) M TLCK对效应细胞进行1小时预处理可完全抑制细胞溶解，仅对靶细胞进行1小时处理则抑制60%。

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J Immunol. 1980 Mar;124(3):1028-33.
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